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KMID : 1098420070150010038
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 1 p.38 ~ p.45
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Effect of M11C (Non-lectin Components) Obtained from Korean Mistletoe on the IL-1beta Secretion from Mouse Splenocytes
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Jun Myung-Ha
Kang Tae-Bong
Chang Sung-Ho
Choi Wahn-Soo
Seong Nak-Sul
Her Erk
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Abstract
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Korean mistletoe (Viscum album L) extract has been found to posses immunoregulating activity. In this study, Korean mistletoe extract, M11C (non-lectin components), was used to know whether this extract activates splenocytes to secret interleukin 1beta(IL-1beta). The splenocytes were treated with M11C, and then collected the supernatant and cell lysate that were prepared to analyze the level of IL-1beta, using ELISA, immunoblotting, and RT-PCR. Maximum effective dose and time of M11C on IL-1beta secretion from splenocytes were 200{mu}g/mell and 8 hours, respectively. Treatment dose and time for the maximum expression of IL-1beta mRNA were 200{mu}g/mell and 4 hours, respectively. Saccharide degradation enzyme Viscozyme L completely blocked the effect of M11C on IL-1beta secretion from splenocytes. As the result, among non-lectin components saccharide could be regarded as a main component for IL-1beta expression from splenocytes.
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KEYWORD
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Korean mistletoe (Viscum album L), non-lectin components, M11C, splenocytes, IL-1beta secretion, IL-1beta mRNA expression
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